Poster Presentation Australasian Cytometry Society 44th Annual Conference and Workshop

Automating antibody titration using a CytoFLEX LX flow cytometer integrated with a Biomek i7 Multichannel workstationand Cytobank streamlined data analysis (24553)

Rita Bowers 1 , Bhagya Wijayawardena 1 , Guilia Grazia 2 , Vashti Lacaille 3 , Matthew Goff 3 , Karen Holdaway 4
  1. Beckman Life Sciences, Indianapolis, USA
  2. Beckman Coulter, Cassina de’ Pecchi, Italy
  3. Beckman Coulter, Miami, , FL, USA
  4. Beckman Coulter, Lane Cove West, NSW, Australia

Flow cytometry is an important research tool for rapidly characterizing cell populations in suspension.Advances in instrumentation and fluorescent reagents have made it possible to perform flow cytometry experiments using high-throughput 96-well plate-based format.Determining the correct concentration/titer of antibody to use in each experiment is a critical quality control parameter. Using too little antibody can result in incomplete labeling, whereas using excess antibody can lead to increased background fluorescence and binding of antibody to low-affinity sites. As performing antibody titrations can be laborious, time consuming and prone to human error, we sought to automate this procedure.
We further streamlined the workflow for antibody titrations using the Cytobank software platform, which can perform both data analysis and Stain Index (SI) calculations within one program.
We present here an automated walk-away research workflow for performing antibody titration experiments for flow cytometry with streamlined data analysis using laboratory solutions available from Beckman Coulter Life Sciences.

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